They include Switzerland-based goalkeeper Sebastian Osigwe, whose father featured for Nigeria at the 1985 U17 World Cup; John Felegha of Belgian club Eupen, Collins Jamilu from Croatia as well as Michael Olaitan, who recently signed for top Greek club Olympiacos.
The squad also includes 18 players from the recent African Youth Championship in Algeria, where Nigeria placed third.
These players include skipper Abduljaleel Ajagun, Enugu Rangers striker Alhaji Gero as well as Olanrewaju Kayode and Umar Aminu, who was Goal King at the 2013 AYC.
Nigeria are drawn against Portugal, South Korea and debutants Cuba at the U20 World Cup in Turkey, which kicks off on June 21 and ends July 13
The full squad:
Goalkeepers: Samuel Okani (Enyimba), Jonah Usman (ABS FC), John Felegha (Eupen, Belgium), Sebastian Osigwe (FC Emmenbrucke, Switzerland)
Defenders: Shehu Abdullahi (Kano Pillars), Moses Orkuma (Lobi Stars), Ikechukwu Okorie (Enyimba), Kingsley Madu (El Kanemi), Collins Jamilu (HNK Rijeka, Croatia), Chizoba Amaefule (Dolphins), Wilfred Ndidi (Nath Boys), Oluwasemilogo Ajayi (Charlton Athletic youth team), Hassan Abubakar (Wikki Tourists)
Midfielders: Agboyi Ovbokha (Bayelsa United), Mathias Samuel (Kano Pillars), Abduljaleel Ajagun (Dolphins), Christian Pyagbara (unattached), Uche Agbo (Enyimba), Michael Olaitan (Olympiacos, Greece)
Forwards: Edafe Egbedi (AGF Aarhus, Denmark), Daddy Moses Simon (Unattached), Lucky Omeruo (Bayelsa United), Chidi Osuchukwu (Dolphins), Bright Ejike (Heartland), Umar Aminu (Wikki Tourists), Olanrewaju Kayode (unattached), Daniel Etor (Akwa United), Alhaji Gero (Enugu Rangers), Sikiru Alimi (Union Bank FC), Samuel Eduok (Dolphins)